| Buga .- Peaje de El Cerrito 1 | 17.26 km | -19 m | -0.0% |
| Brisas del Valle - Sonso | 6.09 km | 22 m | 0.2% |
| Guacarí - El Cerrito | 6.67 km | 11 m | 0.0% |
| Peaje Cerrito - Puente Providencia | 9.85 km | 46 m | 0.4% |
| Crono #2 Gran Fondo 2016 | 9.68 km | 24 m | 0.0% |
| Providencia-Puente Sucromiles | 12.69 km | -40 m | -0.3% |
| Puente Manuelita - Puente Sucromiles | 7.25 km | -23 m | -0.3% |
| Peaje-Puente S/der | 12.81 km | 31 m | 0.2% |
| Peaje VillaRica - Santander | 14.41 km | 58 m | 0.4% |
| Subida la Agustina | 6.90 km | 313 m | 4.5% |
| Via Mondomo | 3.10 km | 147 m | 4.7% |
| Ascenso La Agustina (Mondomo) | 4.61 km | 297 m | 6.4% |
| I-25 Climb | 1.25 km | 97 m | 7.7% |
| Reten Mondomo-Pescador | 10.74 km | 206 m | 1.4% |
| Mondomo - Tunia | 19.75 km | 310 m | 1.3% |
| I 25 Climb | 1.56 km | 82 m | 5.2% |
| I 25 Climb | 2.92 km | 181 m | 6.2% |
| Rio ovejas - segundo ascenso pescador | 4.89 km | 210 m | 4.3% |
| Rio ovejas - Primer ascenso Pescador | 2.82 km | 178 m | 6.3% |
| Ascenso a Pescador (Sentido Cali - Popayán) | 1.29 km | 78 m | 6.0% |
| Pescador-Tunia | 16.84 km | 323 m | 1.1% |
| I 25 Climb | 1.63 km | 108 m | 6.6% |
| I 25 Climb | 1.21 km | 85 m | 6.9% |
| Calle 6 Climb | 1.76 km | 92 m | 5.2% |
| ፒኤንዳሞ ሲልቢአ Climb | 1.63 km | 130 m | 7.8% |
| tunel - el cairo | 1.36 km | 72 m | 4.9% |
| El Cofre - Lácteos | 1.83 km | 59 m | 3.2% |
| I 25 Climb | 1.88 km | 96 m | 5.1% |
| Ascenso Rio Blanco - Sena | 2.45 km | 64 m | 2.5% |
| clarete - puente variante | 1.45 km | 57 m | 3.9% |
| Descenso Sena - Puente Río Cauca | 4.05 km | -116 m | -2.7% |
| Sprint Torremolinos - Puente Campanario | 0.87 km | 14 m | 0.5% |
| El Bohio | 0.88 km | 45 m | 5.0% |
| I 25 # 1 A 2 Climb | 1.19 km | 84 m | 7.0% |
| Bajada Río Guanabanillo | 12.82 km | -578 m | -4.4% |
| Subida de Rosas - Guanabanillo | 6.91 km | 405 m | 5.6% |
| Bajada Parraga | 17.11 km | -840 m | -4.8% |
| Subida Carretera 25 | 4.03 km | 282 m | 7.0% |
| CRI Piedra sentada el Bordo | 9.59 km | -95 m | -0.7% |
| I 25 # 2 Climb | 1.42 km | 102 m | 7.1% |
| chachagui- daza | 17.21 km | 872 m | 5.1% |
| I 25 Climb | 2.82 km | 156 m | 5.5% |
| Retén-Alto de Daza | 2.07 km | 146 m | 6.6% |
| Pana | 2.65 km | 121 m | 4.5% |
| No break (sin tregua) climb | 1.10 km | 134 m | 12.1% |
| I 25 # 1 A 32 Climb | 2.48 km | 113 m | 4.6% |
| San Juan | 7.18 km | 458 m | 6.4% |
| I-25 Climb | 0.49 km | 90 m | 18.4% |
| I 25 # 6 A 36 Climb | 1.53 km | 134 m | 8.7% |
| I 25 # 38 A 62 Climb | 1.27 km | 91 m | 7.2% |
