| Mt Real Climb Heading West | 1.44 km | 59 m | 4.0% |
| Old Healesville Road (from Healesville) | 8.00 km | -90 m | -0.4% |
| Outlook Hill | 1.05 km | 88 m | 8.3% |
| Grin and bear it...you're almost at the glen | 0.80 km | 43 m | 5.3% |
| Killara Roll | 3.71 km | 29 m | 0.6% |
| winery hill | 0.54 km | 5 m | 1.0% |
| Lilydale To Warburton Rail Trail Climb | 0.78 km | -6 m | -0.4% |
| Rail Trail Woori Yallock to Yarra Junction | 8.31 km | 22 m | 0.3% |
| WY to LP dash | 4.78 km | 23 m | 0.4% |
| From the bridge to the lights ! HIll sprint yeeeh !!! | 0.55 km | 6 m | 1.1% |
| Junction millgrove sprint | 4.86 km | 18 m | 0.4% |
| Lowes Road Climb | 0.38 km | 21 m | 5.2% |
| Warburton Hwy Climb | 2.15 km | 83 m | 3.9% |
| The Half Donna / Cement Creek (cycle2max) | 7.13 km | 492 m | 6.9% |
| Mount Donna B- Start to Creek | 1.67 km | 106 m | 6.3% |
| R18blast | 4.44 km | 297 m | 6.7% |
| Donna Buang Rd Climb | 1.41 km | 138 m | 9.8% |
| Donna K3 to K5 | 2.02 km | 133 m | 6.6% |
| Donna Buang Rd Climb | 0.38 km | 93 m | 24.0% |
| Donna Buang Rd Climb | 1.23 km | 128 m | 10.4% |
| Donna K5-K7 Climbing About Now | 2.25 km | 183 m | 8.1% |
| Acheron Way Climb | 1.14 km | 92 m | 8.0% |
| Acheron Way Climb | 0.58 km | 83 m | 14.3% |
| Donna Interval 2 | 5.87 km | 294 m | 5.0% |
| Donna Buang Rd Climb | 1.29 km | 81 m | 6.3% |
| Donna Buang Rd Climb | 1.32 km | 87 m | 6.6% |
| Donna Buang Road Climb | 0.76 km | 97 m | 12.7% |
| Mt Donna Buang Summit Rd Climb | 0.66 km | 87 m | 13.1% |
| Don Rd descent to River | 5.49 km | -403 m | -7.2% |
| Fork to Healesville | 9.47 km | -474 m | -4.9% |
| Don Road Hill Climb | 1.34 km | 20 m | 0.3% |
| SG Don Rd TT | 4.17 km | -70 m | -1.5% |
| Don Rd Dash To The Lights | 2.89 km | -70 m | -2.4% |
| Don Rd Skatepark sprint | 1.14 km | -25 m | -2.0% |
| Myers Ck Road | 8.40 km | 469 m | 5.6% |
| Myers Creek Road Climb | 1.75 km | 98 m | 5.6% |
| Myers Creek Rd true ascent. | 6.76 km | 437 m | 6.5% |
| Leonards Rd Climb | 1.45 km | 102 m | 7.1% |
| Myer's Creek Rd climb 2nd half | 4.44 km | 287 m | 6.6% |
| Myers Creek Rd Climb | 1.27 km | 71 m | 5.6% |
| St Leonards Rd Climb | 1.62 km | 121 m | 7.4% |
| Frozen cash | 3.60 km | -99 m | -2.7% |
| Smiths Ditch | 0.17 km | -17 m | -9.9% |
| Up Smiths Ditch | 0.17 km | 16 m | 9.0% |
| Toolangi to Healesville | 13.51 km | -361 m | -2.7% |
| Chum Creek Descent | 8.19 km | -296 m | -3.6% |
| lower chum descent | 6.27 km | -84 m | -1.3% |
| the last 800 | 0.72 km | -11 m | -1.2% |
| Aquaduct uphill dash | 0.44 km | 7 m | 1.0% |
| Chum down to turn-off | 1.54 km | -51 m | -3.0% |
