| Middle Road Masher - MPH sign to past side road. NOT through stop sign. Done Right. | 0.59 km | 31 m | 5.1% |
| Erie west over I-90 | 1.10 km | -14 m | -1.1% |
| 383 Hill | 0.34 km | 21 m | 6.1% |
| North to Morgan | 3.27 km | -15 m | -0.0% |
| Arena Hill | 0.49 km | 10 m | 2.0% |
| Over the Thruway Hill | 0.81 km | 15 m | 1.7% |
| Bump over Thruway | 0.27 km | 13 m | 4.7% |
| from Morgan to Black Creek | 2.96 km | -22 m | -0.5% |
| Union St. Rip | 1.97 km | -23 m | -0.9% |
| Almost Home | 0.66 km | -10 m | -1.6% |
| 31A-Holley St | 1.62 km | -38 m | -2.3% |
| Redman Rd. - 31A to West Ave. | 2.45 km | -43 m | -1.7% |
| Redman - 4th Section to West | 2.29 km | -38 m | -1.6% |
| 31W- Redman into Holley | 4.86 km | 12 m | 0.1% |
| 237 to Hulberton | 3.12 km | 15 m | 0.4% |
| Hulberton to the Fork | 1.24 km | 21 m | 1.6% |
| Rt 31 country club to transit rd | 2.73 km | -20 m | -0.5% |
| River Ramp | 0.55 km | 14 m | 2.5% |
| Hammer to the Heliport | 3.60 km | 18 m | 0.5% |
| Aerocar Climb | 0.98 km | 50 m | 5.1% |
| TT NOTL | 16.06 km | -88 m | -0.5% |
| Bee in a bonnet | 2.20 km | -7 m | -0.2% |
| floral clock to bridge | 0.52 km | -11 m | -0.6% |
| Queenston to East/West | 9.56 km | -80 m | -0.8% |
| Queenston Descent | 1.16 km | -48 m | -3.5% |
| Niagara PKWY N to East and west line | 4.45 km | 16 m | 0.3% |
| fort george sprint | 1.45 km | -15 m | -0.8% |
| Toronto Lookout Sprint | 0.63 km | -4 m | -0.7% |
| Par 5 Sprint | 0.45 km | -2 m | -0.4% |
| queen street (watch out for tourists) | 2.29 km | 11 m | 0.5% |
| shaw festival to John | 1.33 km | 15 m | 1.0% |
| NOTL to RAINBOW | 21.81 km | 99 m | 0.4% |
| Niagara - Queenston Parkway | 8.40 km | -16 m | -0.1% |
| East and West South to Line 1 | 0.69 km | 4 m | 0.2% |
| Riverbrink Art Museum to Queenston Roundabout | 2.24 km | 82 m | 3.7% |
| Queenston wall | 0.46 km | 26 m | 5.7% |
| Full Queenston | 1.11 km | 59 m | 5.3% |
| Brock's Bite | 0.17 km | 15 m | 8.4% |
| Queenston Hts (to Park) | 1.00 km | 53 m | 5.2% |
| climb-out-of-queenston | 0.98 km | 56 m | 3.5% |
| Queenston Heights (to roundabout) | 0.88 km | 44 m | 5.0% |
| Queenston Heights Lookout (Lower Climb) | 0.37 km | 18 m | 4.8% |
| Queenston Heights Lookout (Upper Climb) | 0.41 km | 20 m | 4.7% |
| Queenston roundabout to Niagara Whirlpool | 6.27 km | 12 m | 0.0% |
| Whirlpool Windup | 0.52 km | 11 m | 1.8% |
| Whirlpool to Clifton | 3.86 km | -46 m | -0.1% |
| Whirlpool Steps | 1.14 km | 18 m | 1.6% |
| Queen of the Mist | 0.84 km | -15 m | -1.8% |
| Pkwy 600 | 0.64 km | -7 m | -0.4% |
| Murray St (Official) | 0.33 km | 25 m | 7.5% |
