| Fucking Homeway | 1.58 km | 8 m | 0.5% |
| hagendorf-spittelberg | 2.32 km | 157 m | 6.8% |
| Tüfelsschlucht | 5.61 km | 331 m | 5.9% |
| Vor Kirche zum Brunne | 5.36 km | 311 m | 5.8% |
| Hägendorf-Teufelsschlucht | 2.84 km | 192 m | 6.7% |
| Oberer Richenwilweg Climb | 1.33 km | 116 m | 8.7% |
| Bärenwil Schlusssprint | 2.21 km | 110 m | 5.0% |
| Bärenwil Climb | 2.02 km | 94 m | 4.7% |
| Bob Run Langenbruck | 1.69 km | -74 m | -4.4% |
| Breitehöchi | 1.75 km | 111 m | 6.4% |
| Sprungschanze - Sprint | 0.46 km | 25 m | 5.4% |
| Breitenhöhe Abfahrt | 4.45 km | -276 m | -6.2% |
| Mümliswil - Ramiswil Kreuzung climb | 4.01 km | 117 m | 2.9% |
| Ramiswil - Scheltenpass (neue Strasse) | 6.87 km | 382 m | 5.5% |
| Guldenthal 31 Climb | 1.46 km | 82 m | 5.6% |
| Scheltenstrasse New Climb | 0.65 km | 78 m | 11.9% |
| Scheltenpass Short | 2.20 km | 190 m | 8.6% |
| Scheltensprint | 0.93 km | 100 m | 10.7% |
| Schelten angel | 0.60 km | 63 m | 10.5% |
| Descente Shelten | 7.04 km | -460 m | -6.5% |
| Peca 2 | 1.45 km | 116 m | 8.0% |
| PECA Climb depuis la Sente | 1.27 km | 101 m | 7.9% |
| Vermes Bächlen | 6.36 km | 231 m | 3.6% |
| PULSE April Challenge 2021 | 3.22 km | 91 m | 2.8% |
| Au Sacy Climb | 0.92 km | 102 m | 11.1% |
| Schmiedli 31 Climb | 3.85 km | 158 m | 4.1% |
| Bächlen Pass | 2.66 km | 128 m | 4.8% |
| Grändel Climb | 0.68 km | 65 m | 9.5% |
| Schmiedli la Tuf | 1.81 km | 66 m | 3.0% |
| PULSE May Challenge 2021 | 5.64 km | -179 m | -2.2% |
| La Tuf Climb | 1.26 km | 66 m | 5.2% |
| Neuhuesli Climb | 0.39 km | 83 m | 21.0% |
| No 30 | 4.54 km | -96 m | -2.1% |
| Moutier Court | 4.15 km | 128 m | 3.1% |
| Bosse de Moutier-Court | 1.68 km | 66 m | 3.9% |
| Rue Des Gorges Climb | 0.95 km | 91 m | 9.5% |
| Col de Pierre Pertuis (Tavannes - Col de Pierre Pertuis) | 2.05 km | 67 m | 3.2% |
| Le Vion 1 Climb | 1.16 km | 113 m | 9.7% |
| Pierre pertuis Nord | 0.95 km | 48 m | 5.0% |
| Pierres DH | 2.43 km | -134 m | -5.5% |
| Sonceboz - La Heutte | 3.61 km | -34 m | -0.7% |
| Rte de Sonceboz Sprint | 1.09 km | -21 m | -1.8% |
| La Heutte Sprint final | 0.57 km | 6 m | 1.0% |
| Péry Sprint final | 0.70 km | -11 m | -1.5% |
| A16 Climb | 0.33 km | 105 m | 30.9% |
| Frinvillier - Route Principale | 0.65 km | 26 m | 3.1% |
| Frinvillier - Route Principale avec tunnel | 0.73 km | 37 m | 5.0% |
| Finvillier au dessus de Bienne | 1.83 km | 78 m | 3.5% |
| Oberer Quai sprint | 0.76 km | -3 m | -0.3% |
| Bahnhofstrasse Sprint | 0.29 km | 4 m | 1.3% |
