| Strada Regionale 68 Climb | 1.26 km | 96 m | 7.7% |
| Roncolla climb Strada Regionale 68 | 1.86 km | 72 m | 3.8% |
| Strada Regionale 68 Climb | 1.96 km | 83 m | 4.2% |
| La Serra climb Strada Regionale 68 | 4.50 km | 131 m | 2.9% |
| Strappettino Castel San Gimignano | 0.24 km | 15 m | 5.8% |
| Campiglia Climb | 1.10 km | 51 m | 4.6% |
| tratto via Volterrana | 0.70 km | -12 m | -1.2% |
| Colle Alta to Colle Bassa descent | 1.43 km | -60 m | -4.2% |
| Volata finale | 0.85 km | 31 m | 3.6% |
| Raccordo Autostradale Siena Firenze Climb | 2.00 km | 84 m | 4.2% |
| Rotatoria Calp Cappuccini | 1.24 km | 45 m | 3.6% |
| Flowershop to Monteriggioni | 5.57 km | -38 m | -0.5% |
| Decathlon - Siena | 15.75 km | 183 m | 0.7% |
| Vanilla/bivioStrove | 1.92 km | -28 m | -1.0% |
| Vanilla/bivioStrove | 1.92 km | -28 m | -1.0% |
| Sp5 time trial | 1.01 km | -8 m | -0.4% |
| Colonna-bv. Bracciano | 5.14 km | 86 m | 1.7% |
| Colonna to Bar | 6.75 km | 87 m | 1.0% |
| Monteriggioni-Siena (via Cassia) | 10.83 km | 175 m | 1.3% |
| Monti roundabout to turnoff | 0.94 km | 28 m | 2.7% |
| pian largo | 1.67 km | 53 m | 3.2% |
| Il Ceppo to Fornacelle | 1.21 km | 44 m | 3.6% |
| Il Ceppo - San Dalmazio | 2.95 km | 98 m | 3.3% |
| ilCeppo-SanMartino | 1.71 km | 80 m | 3.7% |
| San Martino to McDonalds down down down | 2.04 km | -37 m | -0.0% |
| Cassia Nord to McDonalds | 1.97 km | -37 m | -0.1% |
| strappo San Dalmazio | 0.44 km | 24 m | 5.4% |
| Braccio Ingiù ⚗️ | 1.04 km | -37 m | -3.5% |
| Via Fiorentina south | 0.89 km | 20 m | 0.9% |
| Maccy D's bump | 0.50 km | 19 m | 2.3% |
| Porta camollia | 0.93 km | -23 m | -2.0% |
